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Quappenaufzucht of Oophagen histrionica the group with artificial diet

The first experiments were modifications the first reported by R. Bechtel and H. Zimmermann yolk diet. Even with countless variations of additives, however, no significant growth of the tadpoles was reached, and most died at an early stage of development. Yolk was obviously much too high in calories to be digested by the tadpoles. Gradually the food was down to small groups varies from egg yolk and complemented by other additions, the consistency was obtained by adding Carbacol gelatinous .

a feed based on fish eggs then has led to success. The food must be cooked but still fresh daily, requires the use of an analytical balance and the procurement of folic acid and iodate is not without problems (for composition see table). This was a uniform and reach over natural breeding only slightly retarded growth. Unfortunately, it has been found that the problems that the artificial rearing are not resolved.

table: Artificial feeding for Oophagen-tadpoles.

fish eggs (1), frozen and crushed in a mortar, 0.8 ml
amino acids (Eialbuminhydrolysat) (2), powdered, 80 mg folic acid
/ iodate solution (3) 50 mu.l

(1) fish eggs frozen (cod), Ruto BV Zeven Huizen, Holland
(2) Egg Amino, Power Food, Wetzikon, Switzerland
(3) Folic acid 0.01 mg / ml plus potassium 0, 5 mg / ml

The tadpoles were placed individually in PE / PP funnels, which were closed with a close pressed PTFE cones and can be plugged into conical plastic frames in plastic tubs, raised (Fig. 1). Here were the siblings of a clutch arranged side by side. Origin and hatched were recorded. It turned out that developmental disorders in the tadpoles always in a Geschwisterreihe gleichförmig und zeitlich eng korreliert auftraten. Das erschien mir äusserst seltsam. Die Störungen waren:

1. Ein Wachstumsstopp, ohne dass die Quappen eingingen: sie frassen weiter, wenn auch geringe Mengen. Das hätte man noch als Folgeerscheinung eines ungeeigneten Futtes werten können.

2. Sie entwickelten Hinterbeine, aber danach stoppte die Entwicklung, es kam keine volle Farbausbildung mehr zustande. Auch in diesem Fall verendeten die Quappen nicht,
sondern blieben einfach stehen. Eine Aufzucht (etwa 140 Quappen) entwickelte komplett keine Vorderbeine (also nicht SLS).

3. Finally, there were tadpoles that reached full development in spite of the shore and not drowned. In normal development of the shore for the little frogs was trouble, you had time to give a plexiglass top of the funnel to prevent leakage between the hopper and the little frogs were during the 2-3 days under this article, extremely agile and nimble alternated between the water and the hopper back and forth.

1: Oophagen-rearing tadpoles in their hoppers in diluted conical cone tea, water changes every 2 hours to feeding.
The young frogs were reared to 12-15 in 60x40x50 cm lush terrarium. They grew very exciting until the 7th-8. Month, the sudden loss without symptoms of illness began. And again it was the siblings, the absence of time in short succession. So the first example, the yellow bull's eye and a few weeks after the red, were somewhat younger.

2: Oophaga histrionica, morph bull `s eye yellow, about 4 months old.
I set the rearing of tadpoles and then decided to to look into the reasons for the losses. My suspicions were genetic disorders. Gene switch with various internal and external triggers are becoming more numerous known. I had read in earlier posts about Dendroboard SLS, which reported a genetic disorder in SLS. So I took the work of Dr Th Woehrmann. Strangely, this was work - although running in Aachen - here actually gone unheeded. Correct After a meeting with Dr Wöhrmann one must indeed some of the indirectly obtained information (which I have played in the post to SLS), a total one, however a genetic Disorder in SLS go.

A further search for improvement of the artificial diet in the hope of better breeding rates to go by is, in my opinion, useless. I expect that to multiple disorders of the inner and / or external environment Ablesestörungen of genetic information and cause significant improvements in nutrition and welfare will be necessary to meet it. A search for "the" cause will not succeed. Only when the basic search for the possible errors one discovers how long this list is and how much got used to it finds. This has certainly not the first successful artificial Quappenaufzucht contributed decisively (E. Kästner: Mistakes have their value, but only now and then, not everyone drives of India, discovered America).

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the Sunday of the week came in the hip unfriendly

On Sunday came the week unfriendly in the hip. My dust, he said, are more income than your incense scents. But that said, to which the dispute, the law is with us both. Werkeltag you paid for the time to reward me for ages. Henry Schaffer, (1862 - 1937), German writer

I'm wrinkled, put away my book and get out of Tub. After the typical male "T-shirt-not-fresh-trial procedure" I pull at me and storms to the car. Actually I had planned to take the train because it's better for the environment and my wallet, but thanks to the German train, I find it disgusting to use them. Constantly, the train is late, the cars are too full and the timing is just underground. Every 20 minutes is simply not enough. I think we Germans would total more travel by train if they were not small points. Can not be that difficult. While I drive my car at a station I consider myself to compete at the German track. Of course, for the Board, I will eventually result in something. As soon as I had thought this idea over, I let him fall again. Megalomania is called something like that.

I have to think back to earlier. When I was still single. Sometimes I wonder why I almost always have the feeling of sitting in a hole? Why do I think all the time, that I am not complete? "Hey, I am a man, I put the dozen women flat because I need it." I hear myself think and reply right away that I would actually quite stuffy living with someone. Such a common-thing halt.
Despite the almost 3 years I was together with Sandra, I never had this Together-Ding-feeling. The sex was good, at least in the beginning and the unimportant things in life, drink, celebrate and have fun. But more was ever not there. It was especially easy to err.

finally arrived at the garage I try my thoughts mess to put aside and concentrate to park myself on, get out, ride the elevator to the right floor, fetch me a cold Coke, because I like at this time no coffee and sit me in front of my screen. Another day like any other week. Here's a little something to work, because a small talk to work with colleagues and then what. Between what is eaten, or meet up in the break room on a coffee or whatever in my case in a Coke. Strangely, I had
not so many weight problems. 'm Not worried in this regard. No matter. Not important.
At the end of the day I leave the office, sit in my car and go to sports or go directly home. I usually stop at a food temple of my choice. A varied diet is important, so I always alternate between Burger King, McDonalds, Maredo, Giovanni (the local pizza hut) or my favorite Greeks, who also has a wicked Currywurst.

with my healthy food bag I'm in the empty apartment. My only common room is the bedroom. A bed, my ashtray, a TV and now all new: a DVD player. Although I passionately love cooking, I have no direct cooking. The space is still empty. Just as the living room. The bathroom is quite nice and that's it. The living room is my PC, but auf'm ground. Sometimes I lie there and chat. That comes but rarely.

I lay on my bed, grab the remote and watch drop by at Vox. Here again is just my favorite entertainment show, "Have you ever?" Mathias Opdenhövel. Amazing how well some candidates.
I am looking into the telly and eat what I'm always worried and go to sleep. Someday I will awake again. Mist, 23:40 clock. Now I can not sleep again and can not try again Tomorrow morning times before going to bed.
I stuck to my last cigarette and become depressed. No, I will unhappily. I would now like to just turn to somebody, that hug, caress, and perhaps just to be there. But there's just an empty bed.

What can a single man in the week the best way to get through the night and finally fall asleep? Right. Masturbation! One of the few advantages as a single man. You you can when you want a scrub. I taps with a new cigarette in my living room office where the PC is waiting for me. Let's see what I find so everything on the web. After a few hours clicking produced, and a bit annoying gechatte I so go to bed around 2:30 clock. I'm tired and have headaches from cigarettes. Finally sleep. At last Tuesday, a few days until the weekend. Until I finally can with the people. Maybe I meet on Saturday so my dream woman, or at least a great One Night Stand

I feel that is my weekend. Satisfied and with a hopeful smile I fall asleep. My tank is already in the wings. Yeah, I'm in a few hours at Dir

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the morning in the 2001 preface

Yes, I am a sleepy heads. I can also very well be a grumpy in the morning because I wake in the morning to have and do not over-friendly, because I wake up alone in the morning. I'm actually also glad that I wake up in the morning alone. More specifically, I am glad that I said to the normal working days and wake up alone, because I have to work. Must sit on 5 days a week at my slightly chaotic desk and make me worry about any computer problems. Well, is holding my job. Actually, I like my job, after all, I am responsible for what I do, 5 days a week during the day. So why make accusations?
Or even worse how to improve what they want, when I feel at bottom quite well?

course, I also work with typical left-makers, at least I think that this is Links carrier is, but since I now want to not continue it. So I will awake, and even though I basically put an alarm clock, flextime was due.
course there are times where it is absolutely necessary, unfortunately, but a bit of morning stress can not hurt.
So I will awake and see nothing, absolutely nothing. 1.80m wide in my bed I was looking at a half-full ashtrays, no more lies next to me.
Okay, I admit that smoking itself is a necessary part truck already. I belong also to the people you might well see a poster in the city again, with a broad smile and the really big headline: "I enjoy smoking" But
morning in a fairly large bed to wake up overlooking a half-full ashtray is a bit depressing. Especially to this the disgust and "Wow, I'm sloppy" components are added.
is especially since even before smoke in bed yes warned again, but at that moment when I go to bed alone in the evening and I look at my evening television broadcasts, I just want to smoke it. Lazy as I am now times, I have no motivation for each cigarette my chubby hot and really cozy bed to leave. I tell myself in that moment again and again: "Yes, I'm super sloppy and I want to change that." But the very next night you can experience how quickly a person forgets.
Well, I'm so awake, I see the ashtray and the whole evening before hisses over in a fraction of a second.

The whole long evening. This does not end
terrible ending a long evening when I lay in bed for hours and could not sleep because I just do too much. Thoughts are not clear and can not be defined, but quite human.
I say to myself: "Forget it" and shuffle to the bathroom. On the way there I stumble on fast food bags, the night before.
Yeah, after the care of my friendships through joint admissions dauerhocking or in any stores, was yesterday again extreme frustration Fressing announced. Unfortunately, those times have only the fast food chains, especially since you can order in which his food always comfortable and who, without leaving his car.
Brave New World!
ass I am glad that I have turned on the radio. I hate my lousy mood good morning by about funny moderators let it spoil. I am of the opinion that one must live every feeling completely miserable morning by my whims, I'm balanced, at least for the rest of the day and more cheerful.
Especially since nowadays the danger very great is the one on the radio this morning really stupid and completely stupid Hipp-Hopp Drum and Base jaws stupid music gets heard.
A very good example of this would be as meaningful content and powerful songs with scraps of text as follows: Na Na Na Na Na,
i walk around and sing Na Na Na Na Na.
!!!!!! Terrible
That attracts the bad mood in the length and the danger is very great to be able to grasp a friendly thoughts. I lie, then, like every morning, in the tub and read. Most of the so
takes an hour for as long as I need either a chapter to read to an end or to wake up. I know that a 5 minute shower would wake me at once to make, but my ritual would whistle it. I like to lie in my bathtub. I could move anywhere where there is no tub. For a tub is a part of me. If I had no tub, I would probably read to this day, never a book.

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In 2001, when the song Butterfly was on the radio (come my lady come come my lady ...) I had the idea for a book. I found this document on my computer again and would now like to use it here. So here is my preface to the book "links Entity:

So, here we go. I thought me so. "Püh because haste but I was wrong, I thought so on and before I go any further after a synonym for" thought "search, I thought about what I want to think at all.
Actually I'm empty and to think consumed. Just too tired to think straight. I wish I could let myself drop. Just close your eyes and tilted back, in the hope that someone is there. A person who loves me who appreciates me and catches me when I feel bad. A person who is for me means perfection. Not really a person, but my partner.
My so-called "period of life partner."
What a dream! That would be something. I find myself again and again I over the word "of life partner," not as derogatory schmunzle as before, where I've made fun of people who went out together or maybe even got married and where the intention was clear that these early family before the divorce At length at least one pupil to their happiness more to carry out, before all the parties go their own ways. Where the word "of life partners" still meant "Golden Cage" and "dependency".

But the tide has turned. The word "of life partners" for me to get a sound, and indeed a very beautiful sound, but with an annoying hum, because a portion can mean "too short a time, but in general I must say I do not use this word more derogatory think.
the exact opposite ... Why
links Entity?
After a preface, where the question most of the states:
"Huh, what should he do now?" And the smaller part has been made until the morning thoughts on what spiritual value but the man with all his ideological petty bourgeoisie, together with significance in , the society and this takes over the animal kingdom.
Do I want to save all of this ineffable and unbearable tension.
I can really explain with a short sentence, why "Links carrier, Left carrier" means. It took me
pissed!
Just pissed.
I can also put it really only pretending.
It pissed me.
We live in a time where the woman everything is made easy. All this refers to the woman. Everything is tailored for women and men, we remain on track. After
women's movements, their own TV shows, typical male jobs, the Bundeswehr, books for women, and even our holy porn movies.
is Everything "eingefraut" and we men are the ones who have to compete more than ever, and profiling. We women have to prove even more that we do not this crude and tail-controlled granite blocks are the only one goal . Pursue I think it's time to show me that there is another way and it would be quite different from most females think of us. This also we think about love, relationships and happiness. We have feelings and desires and it's not always about a topic. I'll probably come out myself here as huge wimp who is trying this way to tow more women, but far from it. Sure, there are

black sheep among us, but did you also and because of that there are more women than men are the black sheep outnumber women.
Okay, now that was a real girl milk bill, but not quite refutable.
course, I speak here only from me, from my experience and from my point of view, but I hope that perhaps a few females then men see me differently. If not, you now have at least one more jewel in the bookcase, so I had given me with the book cover also very much trouble.

O horror! Horror! Horror Macbeth II, 3

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The beginning ...

.. is hard, but yes you have to start again. So I started again.
I start just to introduce myself and what you want here.

So my first name is not care. I would like first to remain anonymous. You can call me but Harry. No, my name is not Harry S. Morgan and producing porn. I looked last time nor the romantic comedy "When Harry meet Sally ..." and I was kind of a no other name.
So, I'm Harry, and take any car, but I'm in a right relationship crisis. While I try to deal with my crisis, I realized that we all are in a crisis, or let's say almost all. Almost everyone has stress. Whether it is the relationship with partners, to the job or his financial adviser, who will not allow a further overdraft of the account. We all have many and various crises and must be overcome.
Hm, I just noticed a lot of black?

I think not. Maybe you have the Location to be simply aware only. Maybe you have to simply let all times approaching. Maybe you have to be still endure the odd farmer saying about yourself or perhaps you have simply come to life again. Just enjoy times. It has only one life and uh, no, that is now back in the direction of farmer saying.

But back to my or to your crisis. Have you ever found how simple some solutions are and why the solution does not recognize, although it is so close to the resort? Why exactly, and why can be just as many separate and go their separate ways? Did it not work? Was the sex suddenly bad? Was the beer belly of the man but then too big or become the fabric of women has deteriorated too much? Maybe even the evolution has struck, because according to a scientific research, we can love only about 3 years. After that it is habit. The original idea was that we draw after 3 years on and we continue to grow.
Only it's not easy. Condo, 2 cars and Hülsta living room cabinet must first be broken.
you the glasses, the dishes and then I nor the housing problem.

Anyway, I want to try this blog with some answers or solutions to present. I want to show that we too are men such pigs does not necessarily colloquially as always is claimed. Maybe it will be me at all and I have at least one or the other for a few minutes to think or smile to be related. That would be already something.

I look forward to many words and sentences and who may effusions may want to say one or the other comment.
morning comes the first chapter.

Have fun while reading.

Harry without Sally